WebJan 5, 2024 · Stimulated emission depletion nanoscopy (STED) is increasingly applied for the insights into the ultra-structures of organelles in live cells because of the bypassing of the Abbe's optical diffraction limit. Theoretically, with the increase of excitation and depletion laser power, the imaging resolu … WebImaging is performed in an organotypic hippocampal slice culture system, with yellow fluorescent protein (YFP) used as a volume marker for the synaptic structures. In addition, …
Stimulated emission depletion (STED) super resolution …
WebJun 5, 2024 · Understanding the plasma membrane nano-scale organisation and dynamics in living cells requires microscopy techniques with high spatial and temporal resolution, permitting for long acquisition times, and that allow for the quantification of membrane biophysical properties such as lipid ordering. Among the most popular super-resolution … WebOct 31, 2024 · Stimulated emission depletion (STED) microscopy unravels intracellular structures and provides insights into nanoscale domains in cells. Although the image … poorly characterized中文
Possible use of a BGO array for neutron stimulated emission …
WebJul 11, 2024 · The basic approach behind stimulated emission depletion (STED) is straightforward [ 1 ]. It starts where confocal microscopy ends: a small region is excited by a focused laser beam ( Figure 1 A). However, “small,” by the laws of physics, is not an arbitrary term but happens to be about half of the wavelength used. WebDec 14, 2024 · Stimulated emission depletion (STED) nanoscopy is a promising fluorescence microscopy to detect unresolvable structures at the nanoscale level and … WebApr 13, 2024 · They labeled the VIM-Halo fusions with quality comparable to the bench-mark probe 580CP-x and performed well in stimulated emission depletion (STED) microscopy in living cells (Figures 2 and 3). ... The live cell STED imaging in this spectral area is challenging, due to phototoxic effects of the excitation and STED beams “amplified” by … sharelocked